Regulatory

Part:BBa_I12021:Design

Designed by: ryhsiao   Group: Antiquity   (2004-07-26)


Inducible Lambda cI Repressor Generator (Controlled by IPTG and LacI)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The absence of p22 cII (constitutive promoter) will yield a constant promotion of LacI, which inhibits the expression of lambda cI. Once IPTG is introduced into the system, it will bind to the LacI, resulting in expression/promotion of lambda cI via the QPI (Quad Part Inverter). Hence, a switch controlled by IPTG is created.



Source

References